电离辐射通过转化生长因子-β-介导的上皮-上皮转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：聚焦辐射源是否可通过转化生长因子-β（TGF-β）-酪氨酸的表皮-间质转换 (EMT)来促使细胞的侵扰迁往。用于总量2Gy(60)Coγ线照射源自人类文明器官的6种细胞，记录与EMT无关的变化，这包括分别利用显微镜技术，RNA马尔季尼夫卡方律，免疫荧光技术，划痕试制和Transwell的大试制来观察并检查细胞组织形态，EMT标识，侵扰迁往战斗能力等。采用肽联免疫吸附律检查这些细胞之中TGF-β蛋白水准，利用特别抑制剂SB431542来评核TGF-β讯号移动式在辐射源EMT之中的作用。经过总量为2Gy照射的细胞之中存有间叶细胞的解读，与假照射组相比其表皮标识减少，间叶细胞标识降低，同时其侵扰转移战斗能力增强，TGF-β蛋白水准也提高。进一步发现由A549辐射源抑止的EMT可通过对TGF-β讯号抑制遭遇逆转。这些结果表明TGF-β酪氨酸的EMT在辐射源抑止增强细胞侵扰转移战斗能力之中起着关键作用。

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